Copyright ©2023 SMART CAMP
Flagship Project 1
Efficacy CQA
Label-free critical quality attributes for personalized efficacy of cell therapies, including multivariate analysis of biological and biophysical attributes
What ?
Label-free critical quality attributes for personalized efficacy of cell therapies, including multivariate analysis of biological and biophysical attributes.
Why ?
For regulatory approval and clinical use, cell therapies must be demonstrated as both safe and effective. While this expectation of manufacturing standards seems obvious based on Phase I-IV clinical trials for a range of pharmaceutical products and medical devices, for most emerging and approved cell therapies it still remains challenging to relate therapeutic efficacy (in the animal or human patient) to the properties of the cells themselves.
What we do ?
FP1 addresses and identifies CQAs that (i) correlate with therapeutic efficacy and that (ii) are amenable to intermittent or inline monitoring of the therapeutic dose because the CQA measurement does not label, tag, or detectably alter the cells’ therapeutic efficacy. While these CQAs will be specific to a given cell type/source and its intended target indication (disease or injury repair), the general framework of cell population measurement (even at single cell resolution), multivariate data analytics, and validated correlation among biological, biophysical, and therapeutic outcome data is shared. Thus, FP1 will develop and demonstrate this CQA approach for multiple distinct cell types that provide contrast in technical requirements and synergies in CQA validation methodology development, including relatively surface-adherent cells such as mesenchymal stromal/stem cells and relatively non-adherent cells such as T cells.
Highlights
SMART discovers novel method to predict effectiveness of mesenchymal stromal cells (MSCs) for cartilage repair
SMART researchers have developed a new method to assess the potential of mesenchymal stromal cells (MSCs) to repair cartilage. This method is faster (9 days) compared to the current standard (21 days) and uses a simpler 2D culture system The new method analyses specific fingerprint-like patterns or topological defects in MSCs to predict how effective they will be for cartilage repair therapy This new method is non-destructive and also more accurate than existing ones, allowing researchers to identify effective cell batches and enable the development of new and more efficient cartilage regeneration therapies
Publications
Meiqi Kang (SMART), Yanmeng Yang (SMART), Haifeng Zhang (NUS), Yuan Zhang (NUS), Yingnan Wu (NUS), Vinitha Denslin (NUS), Rashidah Binte Othman (SMART), Zheng Yang (NUS/SMART) and Jongyoon Han (MIT/SMART)
Comparative Analysis of Serum and Serum-Free Medium Cultured Mesenchymal Stromal Cells for Cartilage Repair
2024
SMART CAMP Authors
Ching Ann Tee (SMART), Daniel Ninio Roxby (SMART), Rashidah Othman (SMART), Vinitha Denslin (NUS), Kiseer Sideeq Bhat (SMART), Zheng Yang (NUS), Jongyoon Han (MIT/SMART), Lisa Tucker-Kellogg (Duke-NUS) & Laurie A. Boyer (MIT/SMART)
Metabolic modulation to improve MSC expansion and therapeutic potential for articular cartilage repair
2024
SMART CAMP Authors
Brandon Krupczak (MIT), Camille Farruggio (MIT) and Krystyn J. Van Vliet (MIT/SMART)
Manufacturing mesenchymal stromal cells in a microcarrier-microbioreactor platform can enhance cell yield and quality attributes: case study for acute respiratory distress syndrome
2024
SMART CAMP Authors
Ekta Makhija (SMART), Yang Zheng (NUS), Jiahao Wang (NUS), Han Ren Leong (NUS), Rashidah Binte Othman (SMART), Ee Xien Ng (SMART), Eng Hin Lee (NUS), Lisa Tucker Kellogg (Duke-NUS), Yie Hou Lee (SMART), Hanry Yu (NUS), Zhiyong Poon (SGH), Krystyn J. Van Vliet (MIT/SMART)
Topological defects in self-assembled patterns of mesenchymal stromal cells in vitro are predictive attributes of condensation and chondrogenesis
2024
SMART CAMP Authors
Ching Ann Tee (SMART), Jongyoon Han (MIT/SMART), Hoi Po James Hui (NUS), Eng Hin Lee (NUS/SMART), and Zheng Yang (NUS/SMART)
Perspective in achieving stratified articular cartilage repair using zonal chondrocytes
2023
SMART CAMP Authors
Smitha Surendran Thamarath (SMART), Ching Ann Tee (SMART), Shu Hui Neo (SMART), Dahou Yang (SMART), Rashidah Othman (SMART), Laurie A Boyer (MIT/SMART), Jongyoon Han (MIT/SMART)
Rapid and Live-Cell Detection of Senescence in Mesenchymal Stem Cells by Micro Magnetic Resonance Relaxometry
2023
SMART CAMP Authors
Khloe S. Gordon, Taeyoon Kyung, Caleb R. Perez, Patrick V. Holec, Azucena Ramos, Angela Q. Zhang, Yash Agarwal, Yunpeng Liu, Catherine E. Koch, Alina Starchenko, Brian A. Joughin, Douglas A. Lauffenburger, Darrell J. Irvine, Michael T. Hemann & Michael E. Birnbaum
Screening for CD19-specific chimaeric antigen receptors with enhanced signalling via a barcoded library of intracellular domains`
2022
SMART CAMP Authors
Ching Ann Tee, Zheng Yang, Yingnan Wu, Xiafei Ren, Maciej Baranski, Daryl Jimian Lin, Afizah Hassan, Jongyoon Han, Eng Hin Lee
A Pre-Clinical Animal Study for Zonal Articular Cartilage Regeneration Using Stratified Implantation of Microcarrier Expanded Zonal Chondrocytes
2022
SMART CAMP Authors
Chayakorn Petchakupa, Paul Edward Hutchinson, Hui Min Tay, Sheng Yuan Leong, King Ho Holden Li, Han Wei Hou
Label-free quantitative lymphocyte activation profiling using microfluidic impedance cytometry
2021
SMART CAMP Authors
Kerwin Kwek Zeming, Rohan Vernekar, Mui Teng Chua, Kai Yun Quek, Greg Sutton, Timm Krüger, Win Sen Kuan, Jongyoon Han
Label‐Free Biophysical Markers from Whole Blood Microfluidic Immune Profiling Reveal Severe Immune Response Signatures
2021
SMART CAMP Authors
Lingyan Gong, Chayakorn Petchakup, Pujiang Shi, Pei Leng Tan, Lay Poh Tan, Chor Yong Tay, and Han Wei Hou
Direct and Label-Free Cell Status Monitoring of Spheroids and Microcarriers Using Microfluidic Impedance Cytometry
2021
SMART CAMP Authors
Kerwin Kwek Zeming, Ri Lu, Kai Lee Woo, Guoyun Sun, Kai Yun Quek, Lih Feng Cheow, Chia-Hung Chen, Jongyoon Han, and Shir Lynn Lim
Multiplexed Single-Cell Leukocyte Enzymatic Secretion Profiling from Whole Blood Reveals Patient-Specific Immune Signature
2021
SMART CAMP Authors
Ng Ee Xien, Wang Ming, Neo Shu Hui, Tee Ching Ann, Chen Chia-Hung, Van Vliet Krystyn
Dissolvable gelatin-based microcarriers generated through droplet microfluidics for expansion and culture of mesenchymal stromal cells
2020
SMART CAMP Authors
Guoyun Sun, Yao Teng, Zixuan Zhao, Lih Feng Cheow, Hanry Yu, and Chia-Hung Chen
Functional Stem Cell Sorting via Integrative Droplet Synchronization
2020
SMART CAMP Authors
Lu Yin, Zheng Yang, Yingnan Wu, Vinitha Denslin, Chia Chen Yu, Ching Ann Tee, Chwee Teck Lim, Jongyoon Han, Eng Hin Lee
Label-free separation of mesenchymal stem cell subpopulations with distinct differentiation potencies and paracrine effects
2020
SMART CAMP Authors
Conference Presentations
Ching Ann Tee, Daniel Ninio Roxby, Antony Dhasan Josphin Denslin, Lisa Tucker-Kellogg, Laurie A. Boyer
SMART CAMP Authors
2023
Ekta Makhija (SMART), Yang Zheng (NUS), Jiahao Wang (MBI), Han Ren Leong (NUS), Rashidah Binte Othman (SMART), Ee Xien Ng (SMART), Eng Hin Lee (NUS), Lisa Tucker-Kellogg (Duke-NUS), Yie Hou Lee (SMART), Hanry Yu (NUS), Zhiyong Poon (SGH), Krystyn J. Van Vliet (MIT
SMART CAMP Authors
2022
Nicholas Tan (SMART), Kerwin Kwek (SMART), Quek Kai Yun (SMART), Teo Kim Leng (A*STAR BTI), Loberas Mavis Gayle Lumawag (NUS), Steve Oh (A*STAR BTI), Hou Han Wei (NTU) Jongyoon Han (MIT)
SMART CAMP Authors
2022
Wei-Xiang SIN (SMART), Denise TEO (SMART), Kevin S. LEE (Erbi Biosystems Inc), Harry LEE (Erbi Biosystems Inc), Narendra Suhas JAGANNATHAN (Duke-NUS), Rajeev RAM (MIT), Lisa TUCKER-KELLOGG (Duke-NUS), Michael BIRNBAUM (MIT)
SMART CAMP Authors
2022
Yanmeng Yang (SMART), Yingnan Wu (NUS), Jian Xiong Tan (NUS), Eng Hin Lee (NUS), Zheng Yang (NUS)
SMART CAMP Authors
2022
Zheng Yang (NUS), Dahou Yang (SMART), Yingnan Wu (NUS), Vinitha Denslin (NUS), Jaylen Tan (SMART), Eng Hin Lee (NUS), Jongyoon Han (MIT)
SMART CAMP Authors
2022
Wei-Xiang SIN (SMART), Faye CHEUNG (SMART), Sandy LEE (A*STAR SIgN), Dedy SANDIKIN (SMART), Denise TEO (SMART), Faris KAIRI (SMART), Cheng-I WANG (A*STAR SIgN), Michael BIRNBAUM (MIT)
SMART CAMP Authors
2022
Kevin S. Lee (Erbi Biosystems Inc.), Wei-Xiang Sin (SMART), Michael Birnbaum (MIT)
SMART CAMP Authors
2022
Wei-Xiang Sin (SMART), Kevin S. Lee (Erbi Biosystems Inc.), Harry Lee (Erbi Biosystems Inc.), Michael Birnbaum (MIT)
SMART CAMP Authors
2022
Zhi Xian Chan, Linxin Hou, Elizabeth Lee, Ching Ann Tee, and Derrick Yong
SMART CAMP Authors
2022
Kevin S. Lee (Erbi Biosystems Inc, Wei-Xiang Sin (SMART), Harry Lee (Erbi Biosystems Inc.), Michael Birnbaum (MIT)
SMART CAMP Authors
2022
N. Persits (MIT), C.C. Farruggio (MIT), Rajeev Ram (MIT), and K. Van Vliet (MIT)
SMART CAMP Authors
2021
Elizabeth Lee (SMART), Maciej Baranski (SMART), Marvin Chew (SMART), Wei Xiang Sin (SMART), Ka-Wai Cheung (SMART), Lisa Tucker-Kellogg (Duke-NUS), Derrick Yong (A*STAR-SIMTech), Michael Birnbaum (MIT), and George Barbastathis (MIT)
SMART CAMP Authors
2021
Yong, Zixin (SMART), Elizabeth Lee (SMART), Maciej Baranski (SMART), Lisa Tucker-Kellogg (Duke-NUS), and George Barbastathis (MIT)
SMART CAMP Authors
2021
Lee, Elizabeth (SMART), Maciej Baranski (SMART), Zixin Yong (SMART), Lakshmi Venkatraman (SMART), Derrick Yong (A*STAR-SIMTech), and George Barbastathis (MIT)
SMART CAMP Authors
2020