Copyright ©2023 SMART CAMP
Flagship Project 3
Integrated PAT
PAT for cell proliferation and recovery, including inline and intermittent monitoring to promote efficacy and safety attributes for timely cell product release
What ?
Integrated process analytic technologies (PAT) for cell proliferation and recovery, including in-line and intermittent monitoring to promote efficacy and safety attributes for timely cell product release
Why ?
Approaches to monitor and test the CQA – for example identity (phenotype), potency, efficacy, and purity of cells in real-time remains slow and cumbersome. Advancing this requires the development of new chemical, physical, microbiological, mathematical, and risk analysis tools collectively known as integrated process analytics technologies (PAT) so that the very cells that are expanded in vitro and intended to deliver to the patient can be analyzed prior to packaging and delivery.
What we do ?
FP3 develops and demonstrates process analytics technologies (PAT) and feedback control systems to monitor and modulate the appropriate release, recovery, and quantification of proliferating, adherent and suspended cells.
Highlights
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Publications
Ri Lu (NUS), Yan Shan Ang (NUS), Ka-Wai Cheung (SMART), Kai Yun Quek (SMART), Wei-Xiang Sin (SMART), Elizabeth Lee (SMART), Shir Lynn Lim (NUS), Lin-Yue Lanry Yung (NUS), Michael E. Birnbaum (MIT/SMART), Jongyoon Han (MIT/SMART), Lih Feng Cheow (NUS), Kerwin Kwek Zeming (SMART)
iSECRETE: Integrating Microfluidics and DNA Proximity Amplification for Synchronous Single-Cell Activation and IFN-𝜸 Secretion Profiling
2024
SMARTÂ CAMPÂ Authors
Hyungkook Jeon (MIT), Caleb R. Perez (MIT/SMART), Taeyoon Kyung (MIT), Michael E. Birnbaum (MIT/SMART), and Jongyoon Han (MIT/SMART)
Separation of Activated T Cells Using Multidimensional Double Spiral (MDDS) Inertial Microfluidics for High-Efficiency CAR T Cell Manufacturing
2024
SMARTÂ CAMPÂ Authors
Nicholas Tan Kwan Zen (SMART), Kerwin Kwek Zeming (SMART), Teo Kim Leng (A*STAR-BTI), Mavis Loberas (NUS), Jialing Lee (NUS), Goh Chin Ren (SMART), Yang Dahou (SMART), Steve Oh (A*STAR-BTI), James Hui Hoi Po (NUS), Simon M. Cool (A*STAR-IMCB), Han Wei Hou (NTU), Jongyoon Han (MIT/SMART)
Scalable Mesenchymal Stem Cells Enrichment from Bone Marrow Aspirate View Article Online using Deterministic Lateral Displacement (DLD) Microfluidics Sorting
2023
SMARTÂ CAMPÂ Authors
Zheng Li (MIT), Jin Xue (MIT), Marc de Cea (MIT), Jaehwan Kim (MIT/SMART), Hao Nong (GF, Singapore), Daniel Chong (GF, Singapore), Khee Yong Lim (GF, Singapore), Elgin Quek (GF, Singapore) & Rajeev J. Ram (MIT/SMART)
A sub-wavelength Si LED integrated in a CMOS platform
2023
SMARTÂ CAMPÂ Authors
Iksung Kang, Marc de Cea, Jin Xue, Zheng Li, George Barbastathis, and Rajeev J. Ram
Simultaneous spectral recovery and CMOS micro-LED holography with an untrained deep neural network
2022
SMARTÂ CAMPÂ Authors
Sharvaj Kubal, Elizabeth Lee, Chor Yong Tay, Derrick Yong
Multitrack Compressed Sensing for Faster Hyperspectral Imaging, Sensors
2021
SMARTÂ CAMPÂ Authors
Chayakorn Petchakupa, Paul Edward Hutchinson, Hui Min Tay, Sheng Yuan Leong, King Ho Holden Li, Han Wei Hou
Label-free quantitative lymphocyte activation profiling using microfluidic impedance cytometry
2021
SMARTÂ CAMPÂ Authors
Lingyan Gong, Chayakorn Petchakup, Pujiang Shi, Pei Leng Tan, Lay Poh Tan, Chor Yong Tay, and Han Wei Hou
Direct and Label-Free Cell Status Monitoring of Spheroids and Microcarriers Using Microfluidic Impedance Cytometry
2021
SMARTÂ CAMPÂ Authors
Kerwin Kwek Zeming, Ri Lu, Kai Lee Woo, Guoyun Sun, Kai Yun Quek, Lih Feng Cheow, Chia-Hung Chen, Jongyoon Han, and Shir Lynn Lim
Multiplexed Single-Cell Leukocyte Enzymatic Secretion Profiling from Whole Blood Reveals Patient-Specific Immune Signature
2021
SMARTÂ CAMPÂ Authors
Ng Ee Xien, Wang Ming, Neo Shu Hui, Tee Ching Ann, Chen Chia-Hung, Van Vliet Krystyn
Dissolvable gelatin-based microcarriers generated through droplet microfluidics for expansion and culture of mesenchymal stromal cells
2020
SMARTÂ CAMPÂ Authors
Taehong Kwon, Sung Hee Ko, Jean-François P. Hamel, and Jongyoon Han
Continuous Online Protein Quality Monitoring during Perfusion Culture Production Using an Integrated Micro/Nanofluidic System
2020
SMARTÂ CAMPÂ Authors
Conference Presentations
Nicholas Ng Zhi Heng, Kerwin Kwek Zeming, Kai Yun Quek, Francesca Loraine Lim, Yunxin Chen, Chin Ren Goh, Faye Cheung Ka Wai, Sin Wei Xiang, Michael E. Birnbaum, Jongyoon Han
SMARTÂ CAMPÂ Authors
2023
Nicholas Tan Kwan Zen, Kerwin Kwek Zeming, Kai Yun Quek, Francesca Loraine Lim, Yunxin Chen, Chin Ren Goh, Nicholas Ng Zhi Heng, Michael E. Birnbaum, Jongyoon Han
SMARTÂ CAMPÂ Authors
2023
Kerwin Kwek Zeming, Kai Yun Quek, Wei-Xiang Sin, Ka-Wai Cheung, Michael E. Birnbaum, Jongyoon Han
SMARTÂ CAMPÂ Authors
2023
Zhi Xian Chan, Linxin Hou, Elizabeth Lee, Ching Ann Tee, and Derrick Yong
SMARTÂ CAMPÂ Authors
2022
N. Persits (MIT), C.C. Farruggio (MIT), Rajeev Ram (MIT), and K. Van Vliet (MIT)
SMARTÂ CAMPÂ Authors
2021
Elizabeth Lee (SMART), Maciej Baranski (SMART), Marvin Chew (SMART), Wei Xiang Sin (SMART), Ka-Wai Cheung (SMART), Lisa Tucker-Kellogg (Duke-NUS), Derrick Yong (A*STAR-SIMTech), Michael Birnbaum (MIT), and George Barbastathis (MIT)
SMARTÂ CAMPÂ Authors
2021
Yong, Zixin (SMART), Elizabeth Lee (SMART), Maciej Baranski (SMART), Lisa Tucker-Kellogg (Duke-NUS), and George Barbastathis (MIT)
SMARTÂ CAMPÂ Authors
2021
Kubal Sharvaj (NTU), Elizabeth Lee (SMART), Chor Yong Tay (NTU), and Derrick Yong (A*STAR-SIMTech)
SMARTÂ CAMPÂ Authors
2020
Lee, Elizabeth (SMART), Maciej Baranski (SMART), Zixin Yong (SMART), Lakshmi Venkatraman (SMART), Derrick Yong (A*STAR-SIMTech), and George Barbastathis (MIT)
SMARTÂ CAMPÂ Authors
2020